CONTAMINATION OF EXPLANTS OF COFFEA ARABICA L. AS DEPENDING ON THE DISINFECTION USED
P. Valíček, A. K. Wahaishi
Summary
A trial was conducted to study the danger of contamination
of the explants of Coffea arabica L. by solutions used for disinfection. Six
combinations of disinfectant solutions were tested at different exposure times
in meristem culture on three modifications of the LS 65 medium.
The lowest average contamination, 20.59 %, was recorded in group 4, with 10%
Savo (sodium hypochlorite) + O.2 ml Tween 20, on the LS 65 medium + supplements
after Staritsky. At exposure time of 25 min, contamination with this disinfectant
combination was as low as 3.26 %, but at longer exposures the contamination
percentages were higher: 18.52 % at 30 min and as much as 40 % at 35 min. Disinfection
with 2% Savo + 0.2 ml Tween 20, left to act for 108O min, also produced a low
contamination: 22.2 %.
5% chloramine + 0.2 ml Tween was the least suitable disinfectant combination.
It produced an average contamination of 80 %.
Key words:
coffee tree, explants, types of disinfection