IN VITRO MERISTEM CULTURE OF THE COFFEE TREE; THE FORMATION OF THE SHOOTS
P. Valíček, A. K. S. Al Wahaishi
Summary
Two types of meristems were taken from the orthotropic
and plagiotrophic branches of Coffea arahica and C. canephora for in vitro cultivation.
These meristem samples were cultivated on two types of media suitable for the
production of shoots. The parameters under study included the percentage of
contamination plus mortality and the formation of the shoots, i.e. shoot length
and the number and length of the leaves. The meristems taken from the orthotropic
branches grow more vigorously than those taken from the plagiotropic branches.
The apical meristems grow more vigorously than the axillary meristems. Coffea
arabica showed a generally greater growth of the above-ground part than did
the C. canephora.
Key words:
coffee tree, explants