TAUCHEN J., LOJKA B., HLÁSNÁ-ČEPKOVÁ P., SVOBODOVÁ E., DVOŘÁKOVÁ Z., ROLLO A.
Abstract
Calycophyllum spruceanum is an important timber tree species in Peruvian Amazon Basin. Diversity was observed with reference to both morphological traits and genetic variance.
Variance analysis showed significant differences in locality (site of collection) (p < 0.05). Provenance and progenies showed lower variation than locality.
Genetic diversity was assessed using two Internal Transcribed Spacer (ITS) primers ITS1 and ITS4. Fragment sizes ranged between 600-700 bp.
UPGMA analysis separated provenances heterogeneously. The resulting similarity matrix revealed values ranging between 0.672-0.977, with an average of 0.823.
PCA analysis was unable to separate provenances. Our results suggest that morphological diversity is higher than genetic. The discovered genetic diversity under
introduced analyses proved the outcrossing reproduction cycle and population genetics of C. spruceanum. Clearly distinguishable characteristics for each provenance
were not found. Environment factor had a higher impact on phenotype on these studied provenances and localities. Our results are in line with statements of previous
studies on C. spruceanum, suggesting a higher variation within provenances than among provenances. Acquired data will be used for Inter Simple Sequence Repeat (ISSR)
assessment, giving more precise view on genetic diversity of C. spruceanum in Peruvian Amazon.
Key words:
Calycophyllum spruceanum; genetic variation; Internal Transcribed Spacer; morphological description, provenances.