OPTIMIZATION OF THE CONDITIONS FOR TRANSFORMATION AND REGENERATION OF RAPE (BRASSICA NAPUS L) PROTOPLASTS
ELOY FERNANDEZ C., VLCINSKY, M., HROUDA, M.
Abstract
The objective of this work was to find the conditions
for the direct transfer of genes into the rape. As it was shown the critical
point of the whole process of rape transformation was not the own gene transfer
but the ability of the isolated protoplasts to regenerate into intact plants.
We have therefore optimized the individual steps of the entire process which
are inevitable for the direct DNA transfer into the plant protoplasts and we
directed our attention at the conditions of regeneration of plants from protoplasts
in particular.
Key words:
Brassica napus, protoplast, regeneration, transformation,
selection pressure