ORGAN SPECIFITY OF REGENERATION IN EXPLANT CULTURES
OF YACON,
Polymnia sonchifolia Poeppig & Endlicher
ELOY FERNANDEZ, ZDENEK OPATRNY
Abstract
Yacon represents an important natural resource of
polyfruktosans contained mainly in its tubers. Actual level of mono- and polysacharids
can be, besides a number of physiological factors, conditioned by the genotype
as well, characteristic for the given clone which is in vivo maintained with
the aid of rhizoma tubers. A modern alternative of this propagation is represented
by the micropropagation in vitro, from isolated axillary buds. The contribution
compiles the knowledge on the effects of different factors (temperature, composition
of cultivation medium, clone origin) on the effectiveness of micropropagation
and the possibility to derive planting for conventinal cultivation. Following
indicators were observed: process of callogenesis from the explants of different
organ origin (stem, leaves, rhizoma or root tubers), tuberisation in vitro and
particularly the possibility de novo regeneration of stem buds which could be
used to evoce greater variability and to derive production ofselfclones.
Key words:
yacon, micropropagation and tuberisation in vitro.