ROBLES C.H., FERNÁNDEZ C.E.
Abstract
The majority of edible Musaceas does not produce seeds, they
reproduce asexually offshoots or mother shoots. However, the number of offshoots
that can be obtained through the conventional method is relatively small and
also facilitates spreading of pest and diseases. Since the banana plantation
area has been increasing in the last few years, the demand for greater amount
of new plants is required. Recent in vitro technique - microprogation - permits
to obtain a high quantity of plants from little material in a short period of
time. As results, the needs of farmers (bananeros) can be met with a safe material
from both quantitative as well as qualitative point of view.
This research focusus on the optimization of cultivation media for an accelerated
multiplication of banana in vitro conditions. Among other things, we utilized
the influence of some phytoregulators (BAP, NAA) on the shooting and rooting
of in vitro plants. The results of our experiments demonstrated that the optimum
concentration for shooting stimulation of banana crop in vitro conditions (from
the formation of offshoots to explant point of view) is 6 mg/l of BAP in the
MS medium and for rooting 3 mg/l of BAP + 1 mg/l of NAA in MS medium.
Keywords:
Musa sp., banana, micropropagation, in vitro, ex vitro, media
optimization