Differences in Alkaline Phosphatase Activity in Blood Plasma of Cattle from Organic and Conventional Stocks According to Stage of Gravidity

Písek L., Šoch M., Lukešová D.

Abstract
The project was aimed at determining physiological values in activity o ALP (EC 3.1.3.1) in blood plasma of cattle from organic and conventional stocks according to stage of pregnancy. Four blood samples were collected from the two stocks over a period of two years. Blood was collected in the morning and in the afternoon from vena jugularis with one-shot needles into test tubes with heparin. A total of 45 and 43 blood samples were collected in stocks A and B, respectively. The blood serum was obtained by centrifuging blood samples for 10 min at 1500 rpm. To determine ALP [µkat.l-1] activity in blood plasma, Bio-La-Tests (Lachema Brno) and Specol spectrophotometer (Carl Zeiss, Jena) were used. Statistically significant differences in ALP activity between stocks A and B were found (P < 0.05). In stock A, statistically significant differences were found between animals in the second 140 days of gestation and non-pregnant animals (P < 0.01), and animals after calving. Differences between stocks A and B in animals in the second 140 days of gestation were also found (P < 0.05).

Key words:
cattle, organic stock, conventional stock, blood plasma, alkaline phosphatase, gravidity

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