KHOSHKDAMAN M., KAZMPOUR M.N., EBADI A.A., MOSSANEJAD S., PEDRAMFAR H.
Abstract
Foot and sheath rot of rice caused by Pectobacterium carotovorum subsp. carotovorum and Pseudomonas syringae pv. syringae were the important bacterial pathogenof rice in Iran. The disease causes damage on rice which leads to lots of yield in the host plants. During the spring and summer of 2005-2006 different paddy fields regions in Guilan province (Roodsar, Langrud, Lahijan, Rasht, Anzali, Fooman, Soomeesara and Roodbar) were surveyed. Samples were collected from seedling blight and sheath and stalk rot rices. Infected tissues were washed with sterile distilled water and crushed in peptone water. Then 50 µl of the extract were cultured on King's B, NA, CPG and LPGA media containing cyclohexamide (50 µg/ml). After 48 to 72 hours, bacterial colonies were selected and purified. On the basis of morphological, physiological, biochemical characteristics, pathogenicity and PCR with specific primers, the isolates were placed in two groups. The first group was 21 strains that caused foot rot, identified as P. c. subsp. carotovorum. The second group was 22 strains that caused sheath rot identified as P. s. pv. syringae. This is the first report of existence of rice bacterial rot on paddy fields in the Guilan province of Iran.
Key words:
Pectobacterium carotovorum subsp. carotovorum, Pseudomonas syringae pv. syringae. Sheath and foot rot, rice