ABRAHA E., BECHYNĚ M., KLÍMA M., VYVADILOVÁ M.
Abstract
Three factors affecting embryogenic processes in microspore culture of Brassica carinata were tested:
developmental stage of isolated microspores (late uninucleate to early binucleate), level of the elevated temperature
(30; 32 and 25°C) and effect of density 70 000 to 150 000 microspores per 1 ml of the medium. We have developed
an efficient and reliable protocol for Brassica carinata microspore embryogenesis. Our results confirmed
remarkable effect of all examined factors on the formation and further development of microspore derived embryos,
observed in other genotypes from genus Brassica. Optimal values for above mentioned factors were determined in
five various genotypes and could largely help to improve the protocol for efficient and rapid production of
completely homozygous plants of Brassica carinata.
Key words:
Brassica carinata, microspore culture, microspore embryogenesis