ABRAHA E., KLÍMA M., VYVADILOVÁ M., BECHYNÌ M.
Abstract
The effects of colchicine, trifluralin and oryzalin on embryo production and chromosome doubling in embryogenic
microspore cultures of Brassica carinata genotypes were examined. The application of colchicine stimulated the
production of cotyledonary embryos in all genotypes tested when compared to the control variant. Mean rate of
doubled haploid plants from the trifluralin treatment was 86.2%, followed by colchicine in vitro (69.0%), oryzalin
(63.5%) and finally the in vivo colchicine treatment (49.5%). The level of spontaneous diploidization was only
40.2%. The highest frequency of abnormal plants was observed after oryzalin treatment (11.9%), the lowest from
trifluralin (3.5%) and control (4.7%) treatments. The application of 10 ìmol/l trifluralin solution during 18 hours of
microspore culture was found to be the superior method for doubled haploid production. The use of trifluralin to
double chromosomes in microspore culture could be more efficient, cheaper and less toxic substitution of widely
used antimitotic agent colchicine for production of doubled haploid lines also in cultivars and breeding materials of
Brassica carinata.
Key words:
Brassica carinata, colchicine, doubled haploid, microspore culture, oryzalin, trifluralin