KHAYAMIE S., NIKNEJAD KAZEMPOUR N., RABIE B., SASSANIE S., EBADIE A.A.
Abstract
Bacterial canker is caused by the bacterial pathogen Pseudomonas syringae pv. syringae which is present in Iran.
Random amplified polymorphic DNA (RAPD) was used to assess the genetic structure of P. syringae pv. Syringe
populations. In this research, genetic diversity of 50 P. syringae pv. syringae isolates from stone fruit trees,
olive and hazelnut were analyzed. Out of 100 bands, generated with 8 RAPD primers, 72% were polymorphic and scored
as molecular markers. Nei-Li similarity coefficients were computed for every pair of strains from data on fragment
presence or absence. Relatedness between strains was evaluated by UPGMA clustering. The results of genetic analysis
showed that hazelnut isolates, are completely separated from stone fruit isolates. In addition, all of stone fruit
isolate were divided in 3 clusters by RAPD primers. This study shows that strains of P. syringae pv. syringe exhibit
genetic diversity detectable by RAPD analysis, and that molecular and statistical analysis of RAPD fragments can be
used both to distinguish between strains and to determine relatedness between them.
Key words:
stone fruit, Pseudomonas syringae pv. syringae, genetic diversity, RAPD