Slíva Š., Viehmannová I., Vítámvás J.
Abstract
Arracacha (Arracacia xanthorrhiza) belonging to Apiaceae family is one of the most important side crops in Southern
America. It is planted for its storage roots containing valuable starch. The goal of these experiments was to establish
optimal protocol for micropropagation of arracacha. For multiplication phase MS medium, half-strength MS medium and
MS medium supplemented with different concentrations of BAP and NAA was used. Indirect morphogenesis in callus cultures,
induced from petioles, was tested on MS medium containing 2,4-D, KIN and on MS medium without PGR. For rooting of
regenerated shoots MS medium without PGR and media containing NAA, 2,4-D, IAA and AC were used. For multiplication
of culture, MS medium supplemented with combinations of NAA and BAP was found to be the most effective (4.2 ±0.73 new
shoots per explant within four weeks). On the contrary, no shoots were formed on half-strength MS medium. Higher
frequency of callogenesis and shoot regeneration was achieved when explants were cultivated on the medium containing 2,4-D
and subsequently transferred to MS medium without PGR. Low frequency of spontaneous rooting was observed in shoots on MS
medium with/without AC.
Key words:
arracacha; Arracacia xanthorrhiza; micropropagation; callogenesis; organogenesis